Expressions of Sry-related High Mobility Group Box 9 and Gastrokine-1 in Gastric Cancer and Their Relationships with Prognosis / 中国医学科学院学报

Objective To explore the expressions of Sry-related high mobility group box 9(SOX9)and gastrokine-1(GKN1) in gastric cancer tissues and their relationships with clinicopathologic features and prognosis of patients.Methods Immunohistochemistry was used to detect the expressions of SOX9 and GKN1 in 70 cases of gastric cancer tissues and corresponding paracancerous tissues including 27 cases of intestinal metaplasia and 43 cases of normal gastric mucosa. The relationships of SOX9 and GKN1 expressions with clinicopathological features and prognosis were analyzed in gastric cancer tissues.Results The high expression rates of SOX9 in gastric cancer tissues,intestinal metaplasia,and normal gastric mucosa were 92.9%(65/70),77.8%(21/27),and 55.8%(24/43),respectively(=21.722,<0.001). Positive nuclear and cytoplasmic staining was observed. The high nuclear expression rate of SOX9 in gastric cancer tissues was 67.1%,which was significantly higher than those of intestinal metaplasia(37.0%,=0.007)and normal gastric mucosa(23.3%,<0.001). The high cytoplasmic expression rate of GKN1 in normal gastric mucosa was 76.7%,which was significantly higher than those of intestinal metaplasia(44.4%,=0.006)and gastric cancer tissues(37.1%,<0.001). Univariate analysis demonstrated that the nuclear expression of SOX9 in gastric cancer was associated with the degree of tissue differentiation(=0.007),while the cytoplasmic expression of GKN1 was associated with both the degree of tissue differentiation(=0.002)and whether the pathological type was a signet-ring cell carcinoma(=0.009). Furthermore,the nuclear expression of SOX9 was negatively correlated with the expression of GKN1 in gastric cancer(=15.424,<0.001). The 5-year survival rates of patients with high or low nuclear expression of SOX9 were 33.8% and 67.5%,respectively(=0.016).The 5-year survival rates of patients with high or low expression of GKN1 were 60.0% and 35.6%,respectively(=0.044). Further research indicated that 5-year survival rate of patients with high nuclear expression of SOX9 and low expression of GKN1 was 28.8%. Cox multivariate regression analysis showed that TNM stage(stage Ⅱ:=7.435,95%:1.313-42.096,=0.023;stage Ⅲ:=12.214,95%:2.677-55.721,=0.001)and nuclear expression level of SOX9(=3.297,95%:1.199-9.065,=0.021)were independent risk factors for the prognosis of gastric cancer patients.Conclusions Changes in the expressions of SOX9 and GKN1 may be associated with the malignant biological behavior of gastric cancer. SOX9 may be a potential prognostic factor. The combined detection of SOX9 and GKN1 expression and the further study of their molecular mechanism may provide new clues for early diagnosis,targeted therapy,and prognostic prediction of gastric cancer.

Screening of serum proteomic patterns and tissue-specific genes for early liver metastasis of colorectal cancer / 第二军医大学学报

To screen for the serum proteomic patterns and related genes in early liver metastasis of colorectal cancer by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS)and the RT2 Profiler TMPCR Array Human Tumor Metastasis (PAHS-028A) chip, so as to provide theoretical evidence for diagnosis of early liver metastasis of colorectal cancer. Methods The serum proteins of 20 colorectal cancer patients and 20 patients with early liver metastasis of colorectal cancer were detected by WCX 2 chip and SELDI-TOF-MS. PCR gene chips were used to screen the differentially expressed genes between the primary tumor and the liver metastases. Results SELDI-TOF-MS found that, when the M/Z values ranged 2000-30000, the contents of two proteins (3774 and 11851) were significant different in three samples. PCR gene chip found that the expressions of following genes were significantly higher in the primary colorectal cancer specimens than in the liver metastatic nodules: ACTB, APC, CTNNA1, NR4A3, MMP10, CTSL1, RB1, HPSE, ETV4, GNRH1, CDKN2A, KISS1R, IL8RB, ITGA7, ITGB3, DENR, RPSA, CXCR4, MYCL1, NME2, PNN, SMAD4, MMP11, SRC, RORB, SSTR2, SYK, TCF20, MMP3, TIMP2, TIMP3, TIMP4, and TRPM1;and the following genes were significantly higher in the liver metastases than in the primary tumors: MMP9, FN1, CST7, and CCL7. Conclusion SELDI-TOF-MS and gene chip technique can provide a theoretic basis for the mechanism, diagnosis and treatment of early liver metastasis of colorectal cancer.